| SES # | TOPICS | READINGS |
|---|---|---|
| 1 | Course Overview, Enzyme Classification | S&K. Chapter 1. |
| 2 | Enzyme Kinetics (Single-substrate Reactions), Measurement | S&K. Chapter 3.1-3.3.3. |
| 3 | Enzyme Inhibition | S&K. Chapter 3.3.4. |
| 4 | Enzyme Inhibition, pH and Temp Effects | S&K. Chapter 3.3.5. |
| 5 | Non-ideal Enzyme Kinetics | Class notes |
| 6 | Immobilized Enzyme Kinetics - External | S&K. Chapter 3.4. |
| 7 | Immobilized Enzyme Kinetics - Internal | S&K. Chapter 3.4. |
| 8 | Applications of Enzyme Catalysis (Biocatalysis) | S&K. Chapter 3.5, 3.6. Handouts |
| 9 | Microbial Growth | S&K. Chapter 6.1-6.2. |
| 10 | Microbial Growth - Batch Cultivation Profiles | S&K. Chapter 6.1-6.2. |
| 11 | Stoichiometry of Growth, Product Formation | S&K. Chapter 7. |
| 12 | Stoichiometry of Growth, Product Formation (cont.) | S&K. Chapter 7. |
| 13 | Models of Microbial Growth | S&K. Chapter 6.3. |
| 14 | Models of Microbial Growth (cont.) | S&K. Chapter 6.3. |
| 15 | Continuous Culture | S&K. Chapter 6.4. |
| 16 | Continuous Culture (cont.) | S&K. Chapter 6.4. |
| 17 | Bioreactor Design | S&K. Chapter 9.1-9.3. |
| 18 | Bioreactor Design (cont.) | S&K. Chapter 9.1-9.3. |
| 19 | Bioreactor Design (cont.) | |
| 20 | Mass Transfer | S&K. Chapter 6.2.3-6.2.4, 10.2.3. |
| 21 | Mass Transfer (cont.) | S&K. Chapter 6.2.3-6.2.4, 10.2.3. |
| 22 | Heat Transfer, Media and Air Sterilization | S&K. Chapter 10.4. |
| 23 | Scale-up | S&K. Chapter 10.2.4. |
| 24 | Scale-down | S&K. Chapter 10.2.5. |
| 25 | Animal Cell Culture | S&K. Chapter 12. |
| 26 | Animal Cell Culture (cont.) | S&K. Chapter 12. |
| 27 | Mixed Cultures | S&K. Chapter 16. |
| 28 | Mixed Cultures (cont.) | S&K. Chapter 16. |
| 29 | Medium Development | Handouts |
| 30 | Medium Development (cont.) | Handouts |
| 31 | Cellular Engineering - An Engineer's View Towards Recombinant Organisms | S&K. Chapter 14. Handouts |
| 32 | Cellular Engineering/Molecular Biology for Process Engineering | S&K. Chapter 14. Handouts |
| 33 | Cellular Engineering/Molecular Biology for Process Engineering (cont.) | S&K. Chapter 14. Handouts |
| 34 | Enzyme Engineering for Process Improvement | Handouts |
| 35 | Enzyme Engineering for Process Improvement (cont.) | Handouts |
Table of Contents
Study Materials
Readings
 Help support MIT OpenCourseWare by shopping at Amazon.com! MIT OpenCourseWare offers direct links to Amazon.com to purchase the books cited in this course. Click on the Amazon logo to the left of any citation and purchase the book from Amazon.com, and MIT OpenCourseWare will receive up to 10% of all purchases you make. Your support will enable MIT to continue offering open access to MIT courses. |
Required Text
[S&K] Shuler, Michael L., and Fikret Kargi. Bioprocess Engineering: Basic Concepts. 2nd ed. Upper Saddle River, NJ: Prentice Hall PTR, 2001. ISBN: 9780130819086.
Additional Resources
Blanch, Harvey W., and D. S. Clark, eds. Biochemical Engineering. New York, NY: Marcel Dekker Incorporated, 1997. ISBN: 9780824700997.
Bailey, James E., and David F. Ollis. Biochemical Engineering Fundamentals. New York, NY: McGraw-Hill Education, 1986. ISBN: 9780070666016.
Rozzell, J. D. "Commercial scale biocatalysis: myths and realities." Bioorg Med Chem 7, no. 10 (October 1999): 2253-61.