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Biological Engineering II: Instrumentation and Measurement >> Content Detail



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Video Tutorial on the 20.309 AFM


Following the Fall 2006 term, these videos were produced from a series of AFM tutorial sessions led by Maxim Shusteff to prepare other instructors, staff and students for the next offering of 20.309. The tutorials explore the theory and practice of using the lab AFM, which differs somewhat from standard commercial AFM systems.


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Tutorial 1: Introduction to the lab AFM (40 min.)


Tour of the AFM components

Hardware operation: preparing and loading the cantilever and sample; setup and alignment using the laser diffraction pattern

Software operation: overview; interpreting computer and oscilloscope displays

Force spectroscopy introduction: engaging the tip; monitoring cantilever deflection; fine-tuning the setup with force curves

(RM - 56K)
(RM - 220K)
(MP4 - 88.3MB)


Hands-on 1: AFM force spectroscopy (15 min.)


Load cantilever and sample; determine basic features of the sample

(RM - 56K)
(RM - 220K)
(MP4 - 32.7MB)


Tutorial 2: More about force spectroscopy (18 min.)


Collecting and analyzing data

Comparing the 20.309 lab AFM and commercial AFM systems

Overview of elastic modulus curves experiment

Cantilever geometry correction factor (numerical calculation of cantilever bending)

(RM - 56K)
(RM - 220K)
(MP4 - 39.1MB)


Hands-on 2: AFM force spectroscopy (43 min.)


(RM - 56K)
(RM - 220K)
(MP4 - 93.8MB)


Tutorial 3: AFM noise Measurement (16 min.)


Estimating Boltzman's constant

More about cantilever behavior

(RM - 56K)
(RM - 220K)
(MP4 - 35MB)


Hands-on 3: AFM noise measurement (9 min.)


More about preparing and loading cantilevers

(RM - 56K)
(RM - 220K)
(MP4 - 20.9MB)



Background on 20.309 Lab Setups


An Atomic Force Microscope (AFM) For The Instructional Laboratory

Optical Trap



Other Resources


OpenWetWare is an open information-sharing resource among researchers and groups who are working in biology and biological engineering.


 








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