| Module 1: Size and Components of Cells and Implications with respect to Regulation |
| L1 | Introduction: cell constituents, prokaryotes vs. eukaryotes (PDF) |
| L2 | Introduction (cont'd) (PDF) |
| Module 2: Fatty Acid Synthases (FAS), Polyketide Synthases (PKS), and Non-ribosomal Polypeptide Synthases (NRPS) |
| L3 | Fatty Acid Synthase: polymerization, biosynthesis, players, chemistry, structure, chemistry as a paradigm for PKS and NRPS, medical interlude (PDF) |
| L4 | Experimental methods for elucidating FAS structure (PDF) |
| L5 | Chemistry of FAS as paradigm for other molecular machines (PDF) |
| L6 | Secondary metabolism: PKS, NRPS (PDF) |
| L7 | Chemistry of PKS and NRPS: post-translational modification, initiation, elongation, decoration, termination, fidelity (PDF) |
| L8 | Chemistry of PKS and NRPS (cont'd) (PDF) |
| L9 | Chemistry of PKS and NRPS (cont'd with specific examples) (PDF) |
| L10 | Biosynthesis of yersiniabactin and cholesterol (PDF) |
| L11 | Cholesterol biosynthesis (PDF) |
| L12 | Cholesterol regulation and homeostasis (PDF) |
| L13 | Sensing insoluble molecules (PDF) |
| L14 | Module 2: Regulation of the transcription level by insoluble metabolites and Module 3: Translation (PDF) |
| Module 3: Translation: Loading, Initiation, Elongation, and Termination - A Machine in Action; Introduction to G-proteins: Switches or Motors |
| L15 | Translation (cont'd) (PDF) |
| L16 | Elongation, termination, RNA polymerase (PDF) |
| L17 | Chemical methods for studying translation and the ribosome (PDF) |
| L18 | Chemical methods for studying translation and the ribosome (cont'd) (PDF) |
| L19 | Chemical methods for studying translation and the ribosome (cont'd) (PDF) |
| L20 | Isoleucine tRNA synthetase (PDF) |
| L21 | tRNA synthase editing mechanisms; G proteins (EF-Tu/EF-G) (PDF) |
| L22 | G proteins: motors (PDF) |
| L23 | G proteins: switches (PDF) |
| L24 | Peptide bond formation; new technologies using the ribosome (PDF) |
| L25 | Module 3: methods for the incorporation of unnatural amino acids and Module 4: what happens as a protein exits the ribosome? (PDF) |
| Module 4: Crypts and Chambers: Macromolecular Machines involved in Protein Folding and Degradation |
| L26 | Protein folding in vitro (PDF) |
| L27 | Protein folding: in vitro vs. in vivo; degradation (PDF) |
| L28 | Protein folding in vivo (PDF) |
| L29 | Chaperone proteins (PDF) |
| L30 | GroEL/GroES (PDF) |
| L31 | Proteases (PDF) |
| L32 | Proteosome (PDF) |
| L33 | Proteosome (cont'd) (PDF) |
| L34 | Role of Ubiquitin in degradation (PDF) |
| L35 | Degradation through polyubiquitination (PDF) |