| SES # | TOPICS | READINGS |
|---|---|---|
| 1 | Introduction | |
| 2 | Nuclear transfer, part 1 | Wilmut, I., A. E. Schnieke, J. McWhir, A. J. Kind, and K. H. S. Campbell. "Viable Offspring Derived from Fetal and Adult Mammalian Cells." Nature 385 (1997): 810-813. Munsie, M. J., A. E. Michalska, C. M. O'Brien, A. O. Trounson, M. F. Pera, and P. S. Mountford. "Isolation of Pluripotent Embryonic Stem Cells from Reprogrammed Adult Mouse Somatic Cell Nuclei." Curr Biol 10 (2000): 989-992. |
| 3 | Nuclear transfer, part 2 | Barberi, T., P. Klivenyi, N. Y. Calingasan, H. Lee, H. Kawamata, K. Loonam, A. L. Perrier, et al. "Neural Subtype Specification of Fertilization and Nuclear Transfer Embryonic Stem Cells and Application in Parkinsonian Mice." Nat Biotechnol 21 (2003): 1200-1207. Egli, D., J. Rosains, G. Birkhoff, and K. Eggan. "Developmental Reprogramming after Chromosome Transfer into Mitotic Mouse Zygotes." Nature 447 (2007): 679-685. |
| 4 | Embryonic stem cells and adult stem cells | Chambers, I., D. Colby, M. Robertson, J. Nichols, S. Lee, S. Tweedie, and A. Smith. "Functional Expression Cloning of Nanog, a Pluripotency Sustaining Factor in Embryonic Stem Cells." Cell 113 (2003): 643-655. Wagers, A. J., R. I. Sherwood, J. L. Christensen, and I. L. Weissman. "Little Evidence for Developmental Plasticity of Adult Hematopoietic Stem Cells." Science 297 (2002): 2256-2259. |
| 5 | Epigenetics - DNA methylation | Okano, M., D. W. Bell, D. A. Haber, and E. Li. "DNA Methyltransferases Dnmt3a and Dnmt3b are Essential for de novo Methylation and Mammalian Development." Cell 99, no. 3 (October 29, 1999): 247-57. Oda, M., A. Yamagiwa, S. Yamamoto, T. Nakayama, A. Tsumura, H. Sasaki, K. Nakao, E. Li, and M. Okano. "DNA Methylation Regulates Long-range Gene Silencing of an X-linked Homeobox Gene Cluster in a Lineage-specific Manner." Genes & Dev 20 (2006): 3382-3394. |
| 6 | Epigenetics - Histone modifications | Weintraub, H., and M. Groudine. "Chromosomal Subunits in Active Genes have an Altered Conformation." Science 193 (1976): 848-856. Cao, R., L. Wang, H. Wang, L. Xia, H. Erdjument-Bromage, P. Tempst, R. S. Jones, and Y. Zhang. "Role of Histone H3 Lysine 27 Methylation in Polycomb-group Silencing." Science 298 (2002): 1039-1043. |
| 7 | Epigenetics - Reading the epigenome | Weber, M., I. Hellmann, M. B. Stadler, L. Ramos, S. Paabo, M. Rebhan, and D. Schubeler. "Distribution, Silencing Potential and Evolutionary Impact of Promoter DNA Methylation in the Human Genome." Nat Genet 39 (2007): 457-466. Mikkelsen, T. S., M. Ku, D. B. Jaffe, B. Issac, E. Lieberman, G. Giannoukos, P. Alvarez, W. Brockman, T. K. Kim, R. P. Koche, W. Lee, E. Mendenhall, A. O'Donovan, A. Presser, C. Russ, X. Xie, A. Meissner, M. Wernig, R. Jaenisch, C. Nusbaum, E. S. Lander, and B. E. Bernstein. "Genome-wide Maps of Chromatin State in Pluripotent and Lineage-committed Cells." Nature (2007): 553-560. |
| 8 | Field trip and written assignment | |
| 9 | Oral presentations | |
| 10 | Reprogramming by defined factors, part 1 | Davis, R. L., H. Weintraub, and A. B. Lassar. "Expression of a Single Transfected cDNA Converts Fibroblasts to Myoblasts." Cell 51 (1987): 987-1000. Xie, H., M. Ye, R. Feng, and T. Graf. "Stepwise Reprogramming of B Cells into Macrophages." Cell 117 (2004): 663-676. |
| 11 | Reprogramming by defined factors, part 2 | Takahashi, K., and S. Yamanaka. "Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors." Cell 126 (2006): 663-676. Maherali, N., R. Sridharan, W. Xie, J. Utikal, S. Eminli, K. Arnold, M. Stadtfeld, R. Yachechko, J. Tchieu, R. Jaenisch, K. Plath, and K. Hochedlinger. "Directly Reprogrammed Fibroblasts Show Global Epigenetic Remodeling and Widespread Tissue Contribution." Cell Stem Cell 1 (2007): 55-70. |
| 12 | Ethics discussion | Domestic Policy Council. The White House. "Advancing Stem Cell Science Without Destroying Human Life." January 2007. (PDF) |